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Most downloaded papers

The papers listed below are the most frequently downloaded papers for the 12 months to the end of May 2007

Downloads Title / Authors
2097 Review
Capillary electrophoresis: a versatile family of analytical techniques
Graham Kemp
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1525 Review
Industrial products - the return to carbohydrate-based industries
Ariel Louwrier
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775 Review
The application of molecular techniques in environmental biotechnology for monitoring microbial systems
Mark A. Schneegurt and Charles F. Kulpa, Jr.
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714 Review
Applications of site-specific chemical modification in the manufacture of biopharmaceuticals: I. an overview
Roger L. Lundblad and Ralph A. Bradshaw
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569 Review
Colorimetric protein assay techniques
Christine V. Sapan, Roger L. Lundblad and Nicholas C. Price
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548 Review
Bioreactor systems for the production of biopharmaceuticals from animal cells
James N. Warnock and Mohamed Al-Rubeai
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473 Review
Nutrigenomics and nutrigenetics: the 'omics' revolution in nutritional science
Edwin C. M. Mariman
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303 Review
Biomaterials and scaffold design: key to tissue-engineering cartilage
Joanne Raghunath, John Rollo, Kevin M. Sales, Peter E. Butler and Alexander M. Seifalian
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235 Review
Freeze-drying of proteins: some emerging concerns
Ipsita Roy and Munishwar Nath Gupta
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229 Biocrystallography, structure determination and beyond
Timothy J. Wess
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201 Review
Tissue-engineered skin substitutes: from in vitro constructs to in vivo applications
François A. Auger, François Berthod, Véronique Moulin, Roxane Pouliot and Lucie Germain
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191 Review
Computer-aided tissue engineering: overview, scope and challenges
Wei Sun, Andrew Darling, Binil Starly and Jae Nam
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189 Review
Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli
Per Jonasson, Sissela Liljeqvist, Per-Åke Nygren and Stefan Ståhl
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179 Review article
The biology and engineering of stem-cell control
Analeah O'Neill and David V. Schaffer
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176 Escherichia coli minimum genome factory
Hiroshi Mizoguchi, Hideo Mori and Tatsuro Fujio
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171 Review
Advances in microbial amylases
Ashok Pandey, Poonam Nigam, Carlos R. Soccol, Vanete T. Soccol, Dalel Singh and Radjiskumar Mohan
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155 Review
Biotechnological applications for surface-engineered bacteria
Henrik Wernérus and Stefan Ståhl
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152 Review
Lipase assays for conventional and molecular screening: an overview
Rani Gupta, Pooja Rathi, Namita Gupta and Sapna Bradoo
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134 Review
The realm of microbial lipases in biotechnology
Ashok Pandey, Sailas Benjamin, Carlos R. Soccol, Poonam Nigam, Nadia Krieger and Vanete T. Soccol
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133 Review
Glycosylation of Pichia pastoris-derived proteins
Roger K. Bretthauer and Francis J. Castellino
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126 Review
Recombinant production of antimicrobial peptides in heterologous microbial systems
Aaron B. Ingham and Robert J. Moore
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126 Review
Oligosaccharides, neoglycoproteins and humanized plastics: their biocatalytic synthesis and possible medical applications
Jozef Nahálka, Jun Shao and Peter Gemeiner
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126 Running-buffer composition influences DNA-protein and protein-protein complexes detected by electrophoretic mobility-shift assay (EMSA)
Karim Roder and Michael Schweizer
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122 Human adult craniofacial muscle-derived cells: neural-cell adhesion-molecule (NCAM; CD56)-expressing cells appear to contain multipotential stem cells
Andrea C. M. Sinanan, Nigel P. Hunt and Mark P. Lewis
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120 A preliminary investigation demonstrating the effect of quercetin on the expression of genes related to cell-cycle arrest, apoptosis and xenobiotic metabolism in human CO115 colon-adenocarcinoma cells using DNA microarray
Imtiyaz Murtaza, Giancarlo Marra, Ralph Schlapbach, Andrea Patrignani, Marzana Künzli, Ulrich Wagner, Jacob Sabates and Amit Dutt
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120 A general one-step method for the cloning of PCR products
Angelo Bolchi1, Simone Ottonello and Stefania Petrucco
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117 Minireview article
Schizosaccharomyces pombe minimum genome factory
Yuko Giga-Hama, Hideki Tohda, Kaoru Takegawa and Hiromichi Kumagai
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116 Tissue engineering of blood vessels: characterization of smooth-muscle cells for culturing on collagen-and-elastin-based scaffolds
Paula Buijtenhuijs, Laura Buttafoco, Andre A. Poot, Willeke F. Daamen, Toin H. van Kuppevelt, Pieter J. Dijkstra, Rob A. I. de Vos, Lotus M. Th. Sterk, Bob R. H. Geelkerken, Jan Feijen and Istvan Vermes
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115 Minireview
The cryopreservation of human embryonic stem cells
Boon Chin Heng, Lilia L. Kuleshova, Soren Müller Bested, Hua Liu and Tong Cao
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115 Computer-aided tissue engineering: application to biomimetic modelling and design of tissue scaffolds
Wei Sun, Binil Starly, Andrew Darling and Connie Gomez
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111 One-step on-column purification and refolding of a single-chain variable fragment (scFv) antibody against tumour necrosis factor a
Mengyuan Liu, Xiangbin Wang, Changcheng Yin, Zhong Zhang, Qing Lin, Yongsu Zhen and Hualiang Huang
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105 Review
Towards molecular farming in the future: transient protein expression in plants
Rainer Fischer, Carmen Vaquero-Martin, Markus Sack, Jürgen Drossard, Neil Emans and Ulrich Commandeur
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102 Immobilization of bone morphogenetic protein-2 on a nanofibrous chitosan membrane for enhanced guided bone regeneration
Yoon Jeong Park, Kyung Hwa Kim, Jue Yeon Lee, Young Ku, Seung Jin Lee, Byung Moo Min and Chong Pyoung Chung
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102 Review
Nuclear proteins as gene-transfer vectors
Annekathrin Haberland and Michael Böttger
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98 Lipase-catalysed transesterification of soya bean oil for biodiesel production during continuous batch operation
Wei Du, Yuanyuan Xu and Dehua Liu
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96 Inducible Escherichia coli fermentation for increased plasmid DNA production
Aaron E. Carnes, Clague P. Hodgson and James A. Williams
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93 Mini Review
Artificial nerve conduits in peripheral-nerve repair
Ruben Y. Kannan, Henryk J. Salacinski, Peter E. M. Butler and Alexander M. Seifalian
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92 Poly(L-lysine)-modified silica nanoparticles for the delivery of antisense oligonucleotides
Shi-Guo Zhu, Juan-Juan Xiang, Xiao-Ling Li, Shou-Rong Shen, Hong-bin Lu, Jie Zhou, Wei Xiong, Bi-Cheng Zhang, Xin-Min Nie, Ming Zhou, Ke Tang and Gui-Yuan Li
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91 Computational-fluid-dynamics (CFD) analysis of mixing and gas–liquid mass transfer in shake flasks
Hu Zhang, Wellae Williams-Dalson, Eli Keshavarz-Moore and Parviz Ayazi Shamlou
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90 Differential analysis of secondary metabolites by LC-MS following strain improvement of Streptomyces lydicus AS 4.2501
Xiao-Bing Li, Bin Qiao and Ying-Jin Yua
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87 Shear-stress preconditioning and tissue-engineering-based paradigms for generating arterial substitutes
Mohamed Baguneid, David Murray, Henryk J. Salacinski, Barry Fuller, George Hamilton, Michael Walker and Alexander M. Seifalian
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87 Novel inhibitors of fatty-acid synthase from green tea (Camellia sinensis Xihu Longjing) with high activity and a new reacting site
Rui Zhang, Wenping Xiao, Xuan Wang, Xiaodong Wu and Weixi Tian
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87 A detailed understanding of the enhanced hypothermic productivity of interferon-g by Chinese-hamster ovary cells
Stephen R. Fox, Hong Kiat Tan, Mei Chee Tan, S. C. Niki C. Wong, Miranda G. S. Yap and Daniel I. C. Wang
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86 Review
Towards molecular farming in the future: moving from diagnostic protein and antibody production in microbes to plants
Rainer Fischer, Jürgen Drossard, Ulrich Commandeur, Stefan Schillberg and Neil Emans
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85 Agrobacterium-mediated delivery of marker genes to Phanerochaete chrysosporium mycelial pellets: a model transformation system for white-rot fungi
Krishna Kant Sharma, Shefali Gupta and Ramesh Chander Kuhad
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85 Review
Towards molecular farming in the future: using plant-cell-suspension cultures as bioreactors
Rainer Fischer, Neil Emans, Flora Schuster, Stephan Hellwig and Jürgen Drossard
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84 Reduction of flatus-inducing factors in soymilk by immobilized ?-galactosidase
Pullakhandam Raghu, Punjal Ravinder and Bhattiprolu Sivakumar
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84 Review
Design and production of recombinant subunit vaccines
Marianne Hansson, Per-Åke Nygren and Stefan Ståhl
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83 Expression of the Blomia tropicalis paramyosin Blo t 11 and its immunodominant peptide in insect cells
Audrey S. M. Teo, John D. A. Ramos, Bee Wah Lee, Nge Cheong and Kaw Yan Chua
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80 The effect of shear stress on human endothelial cells seeded on cylindrical viscoelastic conduits: an investigation of gene expression
Dina S. Vara, Geoffrey Punshon, Kevin M. Sales, George Hamilton and Alexander M. Seifalian
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