 |
|
|
Medline/PubMed Citation?|?Related Articles in PubMed?|?Download to Citation Matcher??
|
|
Biotechnol. Appl. Biochem. (2007) Immediate Publication, doi:10.1042/BA20060208 
|
|
|
| Molecular construction of bionanoparticles: Chimeric Simian Immunodeficiency - Human Immunodeficiency nanoparticles with minimal viral protein content |
| Maria JL Costa, Lu?sa Pedro, Ant?nio P Alves de Matos, Maria R. Aires-Barros, Jos? A Belo, Jo?o Gon?alves and Guilherme N.M. Ferreira |
|
| Centre for Molecular and Structural Biomedicine, University of Algarve, FARO, PT 8000, Portugal.
|
|
Virus-like particles (VLPs) are promising delivery vectors for molecular therapy since they combine the major advantages of viral vectors with significantly fewer viral vector disadvantages. This paper describes the molecular construction of chimeric virus-like particles based on minimal Simian Immunodeficiency Virus (SIV) and Human Immunodeficiency Virus type 1 (HIV1) components. A chimeric protein was constructed by fusion of SIV matrix protein (p17) and HIV1 p6 protein, and we demonstrated that the chimeric protein assemble as 80nm nanoparticles containing ~7700 chimeric protein units. Chimeric VLPs are release from 293T cells and are fully encapsulated with lipid membrane. Chimeric VLPs are produced at 3.7 fold higher levels when compared with SIV p17 VLPs due to duplication of a PTAP domain previously shown as essential for virus particle release. The chimeric VLPs constructed in this paper were efficiently pseudotyped with VSV.G protein as shown by immunoprecipitation assays.
doi:10.1042/BA20060208
Received 23 October 2006/28 February 2007; Accepted 29 March 2007
Published as Immediate Publication 29 March 2007
|
|
|
