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Biotechnol. Appl. Biochem. (2007) Immediate Publication, doi:10.1042/BA20060205 
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| Sulphite reductase mediated synthesis of gold nanoparticles capped by phytochelatin |
| Suresh Anil Kumar, Majid Kazemian Abyaneh, S. W. Gosavi, Sulabha K. Kulkarni, Absar Ahmad and Mohammed Islam Khan |
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| Biochemical Sciences Division, National Chemical Laboratory, PUNE, Maharashtra 411008, India.
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An enzymatic synthesis route to peptide-capped gold nanoparticles has been developed. Gold nanoparticles were synthesized using ?-NADPH dependent sulphite reductase and phytochelatin in vitro. The gold ions were reduced in the presence of enzyme sulphite reductase, leading to the formation of a stable gold hydrosol of dimensions 7-20 nm and were stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis optical absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes, sizes as well as their separation.
doi:10.1042/BA20060205
Received 18 October 2006/3 January 2007; Accepted 12 February 2007
Published as Immediate Publication 12 February 2007
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